{"id":9088,"date":"2018-08-07T21:34:55","date_gmt":"2018-08-07T13:34:55","guid":{"rendered":"https:\/\/www.quarkbiosciences.com\/blog\/miprimer-an-empirical-based-qpcr-primer-design-method-for-small-noncoding-microrna\/"},"modified":"2021-05-24T15:29:59","modified_gmt":"2021-05-24T07:29:59","slug":"miprimer-an-empirical-based-qpcr-primer-design-method-for-small-noncoding-microrna","status":"publish","type":"post","link":"https:\/\/www.quarkbiosciences.com\/tw\/blog\/miprimer-an-empirical-based-qpcr-primer-design-method-for-small-noncoding-microrna\/","title":{"rendered":"miPrimer: an empirical-based qPCR primer design method for small noncoding microRNA"},"content":{"rendered":"<section class=\"wpb-content-wrapper\"><p>[vc_row disable_element=&#8221;yes&#8221;][vc_column][vc_single_image image=&#8221;8076&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Because of miRNAs\u2019 short length, the design of miRNA primers for PCR amplification remains a significant challenge. Adding to the challenge are miRNAs similar in sequence and miRNA family members that often only differ in sequences by 1 nt. This publication describes a novel empirical-based method, miPrimer, which greatly reduces primer dimerization and increases primer specificity by factoring various intrinsic primer properties and employing four primer design strategies. miPrimer-designed primers are capable of discriminating among members of miRNA families, as validated by qPCR assays using Quark Biosciences\u2019 platform. This publication shows that miPrimer provides a cost-effective and valuable tool for designing miRNA primers.<\/p>\n<p>Check out the full publication here:\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5824350\/\">miPrimer: an empirical-based qPCR primer design method for small noncoding microRNA<\/a>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/section>","protected":false},"excerpt":{"rendered":"<p>[vc_row disable&#8230;<\/p>\n","protected":false},"author":1,"featured_media":8759,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[165],"tags":[173,302],"class_list":["post-9088","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-quick-updates-tw","tag-mirna-tw","tag-publications-tw"],"_links":{"self":[{"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/posts\/9088","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/comments?post=9088"}],"version-history":[{"count":2,"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/posts\/9088\/revisions"}],"predecessor-version":[{"id":9273,"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/posts\/9088\/revisions\/9273"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/media\/8759"}],"wp:attachment":[{"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/media?parent=9088"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/categories?post=9088"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.quarkbiosciences.com\/tw\/wp-json\/wp\/v2\/tags?post=9088"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}